FastQCFastQC Report
Mon 27 Feb 2023
SRR3126912.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126912.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130370
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52660.4658651591956616No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41550.36757875739802015No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG27260.24115997416775037No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG26750.2366481771455364No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC25960.2296593150915187No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT25690.22727071666799364No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTAGCGGT21490.1901147411909375No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21130.1869299432929041No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC17790.15738209612781656No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA16810.14871236851650343No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT12950.11456425772092324No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAC201.8385488E-593.993154
GCTACGA3500.084.593834
AGGGCTA4950.084.4988865
CGTTTTT28800.083.979841
ACGGGAT3200.083.712654
AGGGCGT1350.083.549465
AAGGGAC9000.083.027284
CGAAGGG8900.082.929742
ATAGCGG2500.082.816541
AACGCGG1600.082.345991
CGGGTAG800.082.2445
CGCTACG3600.082.2443
AGGGCAT10500.081.4607245
CTACGAC3650.081.117375
AGTACGG1400.080.665461
TACGACC3700.080.021196
TCCGCTA3850.079.443261
TAACGGG2550.079.2736742
CGTAGGG2550.079.2736742
GCGAAGG4100.079.1898651
Produced by FastQC (version 0.11.5)