FastQCFastQC Report
Mon 27 Feb 2023
SRR3126911.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126911.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185440
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55190.46556552841139154No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45380.3828114455392091No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG30540.2576258604400054No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG28740.2424416250506141No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC27370.23088473478202184No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26000.2193278445134296No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21210.17892090700499394No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19300.1628087461195843No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTAGCGGT17750.1497334323120529No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA16980.1432379538399244No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT13710.11565325954919692No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCGA156.905123E-493.990219
CGTTTTT29500.083.87251
CGAAGGG8550.082.99722
CGTAGGG3400.082.932542
AGGGCAT10900.082.780375
ATAGCGG1650.082.674181
AGGGCAC7450.081.3740845
AACGAGG3950.080.978111
CGCTACG3600.080.936013
AGGGATG19100.080.703645
AGTAAGG8000.080.553871
TAGCAGG3550.079.502131
GGGCGAT4850.079.455646
GGTAAGG7000.079.29391
TAGGGCG3050.078.581984
AAGGGCA14900.078.220054
AGAGGGC10550.077.953973
TAGAGGG16850.077.534952
TAGCGGG4850.077.51772
ACGGGAT3400.077.403714
Produced by FastQC (version 0.11.5)