FastQCFastQC Report
Mon 27 Feb 2023
SRR3126910.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126910.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2902677
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292291.0069670169984466No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48620.1675005520765831No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC48340.1665359252855209No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39940.13759712155365547No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT34100.11747776276864426No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC32380.11155219819497655TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG30550.10524767309624875TruSeq Adapter, Index 15 (95% over 21bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG30260.1042485953483629No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG30200.104041889607421TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103450.086.654681
ATAGCGG5000.080.165831
AGGGATG43800.075.855475
ACGGGAT8850.074.0187454
ACGGGTA4150.072.677854
CGTAGGG8550.071.656392
AGTAAGG17600.071.538361
AGGGCGA15400.071.4063345
TAAGGGA25400.071.2471853
GGTAAGG18500.070.607111
GGGCGAT13400.070.490876
TAGGGCA16800.070.410054
TAGCGGG14700.069.5696952
TACGGGT2850.069.4503863
GAGGGAT36400.069.395874
GAGGGTA18100.068.998154
CGAAGGG27450.068.846162
ATAGAGG20700.068.570371
AGGGAAT37850.068.535375
AGTAGGG45050.068.4164662
Produced by FastQC (version 0.11.5)