FastQCFastQC Report
Mon 27 Feb 2023
SRR3126909.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126909.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2906432
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286460.9856070948847248No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47890.16477247704401823No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC47100.16205436769207054No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA38950.13401311298526852No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC32670.11240586395965912TruSeq Adapter, Index 21 (95% over 21bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT32350.11130485763988286No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG29890.10284087155660274TruSeq Adapter, Index 15 (95% over 21bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG29080.10005394930966904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104850.086.2387541
ACGGGTA3200.077.8375244
CGTAGGG8050.075.9389652
ATAGCGG5600.074.807571
TAAGGGA28100.074.759153
AGGGATG45650.074.432165
ATAGAGG20400.072.912251
ACGGGAT7900.072.576474
GGGCGAT10850.071.902096
AGGGTAC14500.071.628755
CGAAGGG26350.071.561752
AGGGAAT37150.071.2217565
TGTAGGG20950.071.152882
TAGGGCA17250.070.562484
AAGGGTA20100.070.3774644
AGTAAGG17850.069.6160351
GGTAAGG17550.069.1968151
AGGGCAT25650.069.0743945
ATAGGGC19900.068.4869843
AGAGGGC37000.068.46213
Produced by FastQC (version 0.11.5)