FastQCFastQC Report
Mon 27 Feb 2023
SRR3126907.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126907.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934696
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298071.5406554828252088No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC44200.22845966498095824No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43130.2229290803309667No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA37280.19269177173054577No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG33260.17191331351282063TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT31530.1629713402002175No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG28170.14560427064510395TruSeq Adapter, Index 14 (95% over 21bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT21820.11278257669421966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT111100.087.881561
AGTAAGG10650.076.2131961
ATAGCGG4300.074.6262741
ATAGGGC9550.074.068743
GGTAAGG10600.072.565971
ATAACGG1900.072.027071
AGGGCAT18100.071.1379245
GAATAGG12200.070.785221
TAGCGGG11100.070.5250552
CGTAGGG4750.070.489282
AGGGTAC7400.070.488855
AGTAGGG28300.069.487712
AAGGGTA12000.068.770774
TAGGGAC8650.068.691274
TAGAAGG15950.068.344351
AGAGGGC22250.068.245333
AGGGACT30900.067.9795465
CGAAGGG17950.067.781882
TAGAGGG33350.067.449842
ATGAGGG27150.067.3938452
Produced by FastQC (version 0.11.5)