FastQCFastQC Report
Mon 27 Feb 2023
SRR3126906.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126906.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948310
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276641.4198972442783746No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC44360.22768450605909737No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42580.21854838295753756No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36210.18585338062218026No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG33150.1701474611329819TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT31570.16203786871699064No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG27230.13976215284015378TruSeq Adapter, Index 14 (95% over 21bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT22020.11302102848109387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105350.086.870071
ACGGGTA1650.074.056224
CGTAGGG5350.073.824132
AGTAAGG9650.073.62661
AGGGCAT15200.072.969365
ACGGGTC2150.072.135264
AGGGTAC8150.072.081635
TAGGGCA9650.072.078634
ATAGGGC9100.071.27053
ATAGCGG4250.070.855931
GAATAGG10350.070.010871
AGAGGGC25000.069.9318543
AGTAGGG29250.069.764852
ACGGGAT4050.069.62554
AATAGGG25500.069.329882
ATAGGGT8150.069.198363
TAGCGGG10000.069.1178362
CGAAGGG19900.068.756332
AAGGGTA10900.068.555574
AGGGATG18850.068.314255
Produced by FastQC (version 0.11.5)