FastQCFastQC Report
Mon 27 Feb 2023
SRR3126905.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126905.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073976
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297531.434587478350762No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC46490.22415881379533806No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44090.21258683803476994No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36880.17782269418739657No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG35040.16895084610429437TruSeq Adapter, Index 14 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT33550.16176657781960832No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG29900.144167531350411TruSeq Adapter, Index 14 (95% over 21bp)
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT23280.11224816487751063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115150.086.827451
ATAGCGG4600.080.803721
GAATAGG11650.073.0995561
AGGGCAT18400.072.790685
AGTAAGG10750.072.216691
ACGGGTA1650.071.202354
GTAGGGC8550.070.352923
ATAGGGC9350.069.862073
TAAGGGA14400.069.837633
TAGCGGG11200.069.651152
GTAGAGG15600.069.368991
TAGAGGG37350.069.326492
TAGTAGG9050.069.145691
TATAGGG15500.068.8228152
CGTAGGG5750.068.651452
AAGAGGG65700.068.594852
AGTAGGG31400.068.395072
AGAGGGC24150.068.3011863
AGGGACT32500.068.250745
AATAGGG27150.068.19692
Produced by FastQC (version 0.11.5)