FastQCFastQC Report
Mon 27 Feb 2023
SRR3126904.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126904.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2778620
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175800.6326881689471752No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT68510.2465612426312342No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA47360.17044432128178738No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC39570.14240882164527716No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG36620.13179204065327393No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT35730.12858901181161872No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG33230.11959173978449734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA3500.086.238984
CGTTTTT66000.081.942071
ATAGCGG6000.077.079891
ATAGGGC16000.075.459113
CGTAGGG7500.074.2054752
AAGGGTA16200.073.071894
AGGGCAT21250.072.976135
AGAGGGC36800.072.408963
TAGGGCG5150.072.34554
AGTAGGG41400.072.227752
AGGGATG33100.071.553095
AGTAAGG16350.071.292761
TAGGGCA14750.070.982714
AGGGTAT14300.070.652625
TGTAGGG18750.070.432312
AGGGTAC11950.070.390015
CGAAGGG27500.069.974962
TAACGGG9400.069.743242
ATAGGGA27100.069.6117253
GTACGGG7600.069.505572
Produced by FastQC (version 0.11.5)