FastQCFastQC Report
Mon 27 Feb 2023
SRR3126903.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126903.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2776354
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167980.6050381183379353No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT66620.2399549913303563No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA46250.16658538500493814No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC40420.14558662187891025No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG37310.13438488031425389No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT35470.12775748337567905No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG31340.11288185872550835No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA2850.084.099064
CGTTTTT63850.083.5037841
CGTAGGG8300.075.899892
ATAGCGG7300.075.422371
ATAGGGC15450.074.5252463
AAGGGTA18050.074.465424
TAACGGG9550.072.3649752
AGGGTAC11000.072.203765
AGTAAGG15400.071.809891
CTAACGG2700.071.459051
TAGGGCG5500.070.9220354
TAAGGGA23200.070.697383
TAAGAGG21950.070.5340041
TAGCGGG14800.070.518932
AGGGCAT22700.069.977225
AGAGGGC35850.069.741023
CGAGGGA13500.069.624493
AGGGATG30850.069.618855
TATAGGG20600.069.606062
CGAAGGG27050.069.171992
Produced by FastQC (version 0.11.5)