FastQCFastQC Report
Mon 27 Feb 2023
SRR3126902.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126902.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2899362
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176120.607443982503737No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT68590.23656928662236726No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA47840.1650018176412604No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC43960.15161956320045583No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG41300.14244513103227538No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG35190.12137152932265788No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT34810.12006089615577496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67800.081.723151
TAGGGCG5300.079.803684
AAGGGTA17950.075.1404344
CGAAGGG27950.074.486612
CGTAGGG7800.074.1082842
ATAGGGC15500.073.070423
TAGGGCA14050.071.580344
ATAGAGG21000.071.2863161
GAATAGG17550.070.814991
AGGGTAC12750.070.772135
AGTAAGG17800.070.613811
ACGGGAT6200.070.493254
ATAGCGG6900.070.2726751
TATAGGG21250.069.663922
TAGCGGG17200.068.8538742
AGTAGGG46850.067.809942
AGTACGG3750.067.789261
AGAAGGG100800.067.649282
TAAGAGG22750.067.458171
AGGGCAT22400.067.348565
Produced by FastQC (version 0.11.5)