FastQCFastQC Report
Mon 27 Feb 2023
SRR3126901.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126901.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952947
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143410.7343261235455955No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA47050.24091795629886525No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT30100.15412604643136757No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG27020.13835500912211135No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG25420.1301622624679523No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC25400.1300598531347753No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG25330.12970142046865582No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23970.1227375858126206No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22910.11730989115424023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55550.085.276471
CGTAGGG6550.076.311052
ACGGGAT5650.075.945954
TACGGGT1500.075.445213
AGTAAGG14800.074.280321
ACGGGTA2550.073.96594
AACGAGG7550.073.117021
AGGGATG27950.072.632715
TACGACC4050.071.939436
AGACGGG12350.071.78172
AGTAGGG30600.071.34812
TAGCGGG9150.071.118212
TAAGGGA17250.071.071573
ATAGAGG11300.070.982291
GTACGGG4800.070.7317052
CGAAGGG18800.070.73172
CGAGGGA9850.069.892143
AAGAGGG67150.069.6608052
AGTACGG2450.069.329161
GGGGGGA356300.069.163536