FastQCFastQC Report
Mon 27 Feb 2023
SRR3126899.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126899.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036305
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143470.7045604661384223No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA47230.23193971433552438No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT29660.14565597982620482No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC28010.1375530679343222No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG27990.13745485082048123No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG27280.13396814327912568No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25870.12704383675333508No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24180.11874449063377047No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22320.10961029904655738No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59200.085.620271
ACGGGAT5200.080.433994
CGTAGGG5700.079.149712
AGGGATG25250.075.93675
AGTAAGG12200.073.681091
AGTAGGG31850.073.1855242
GGTAAGG11600.072.6235661
CGAAGGG19500.072.300212
GACCGAT1450.071.302978
GTAGGGC9100.071.267363
TATAGGG17100.070.630122
ATAGCGG4100.070.0210341
TAAGGGA16250.069.69743
ATAGAGG13650.069.646811
AGGGCAC12650.069.099575
GGGCGAT7350.069.0540856
GGGGGGA367200.068.777746
ATGAGGG32050.068.76982
GTAAGGG32150.068.555892
AGGGCAT16800.067.975115