FastQCFastQC Report
Mon 27 Feb 2023
SRR3126898.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126898.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651676
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122470.7414892509184611No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41310.25010958565723546No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT24870.15057432571521293No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21870.13241095711265405No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20350.12320818368735757No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG19520.11818298504064963No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18520.11212852883979665No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG17840.10801149862321666No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC17790.10770877581317401No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT49150.083.6857151
ACGGGAT4400.080.348324
CGTAGGG4700.079.2314152
GTACGGG3800.078.149312
TAGGGCG3600.074.634664
AGGGATG21150.074.434235
AGGGTAC5550.073.6655655
CGAAGGG15850.073.160062
AAGGGAC14300.070.871334
AGTAAGG10950.070.646091
ATAGCGG2950.070.354051
TAGAGGG28400.070.042612
TAAGAGG12050.069.677311
AGTAGGG24900.069.665322
AGGGCAT14850.069.620075
AGAGGGC18500.069.559933
GGGGGGA301100.069.3587956
TAAGGGA13800.069.34023
TGTAGGG12850.068.964072
GGTAAGG8900.068.898811
Produced by FastQC (version 0.11.5)