FastQCFastQC Report
Mon 27 Feb 2023
SRR3126896.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126896.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722863
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123880.7190356981373446No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42520.2467984976170479No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT25310.1469066315777865No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21450.12450206429646467No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG21290.12357337756977774No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20750.12043905986720942No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20050.11637605543795414No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC18900.10970111958989194No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG18620.10807591781818984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50550.083.306611
CGTAGGG5900.081.240162
ATAGCGG4300.077.6904141
AGGGATG23650.077.295415
CGAAGGG17350.073.399392
GTAGGGC8900.073.3917853
ACGGGTA1800.073.098454
AGTAGGG26750.073.0789262
TAGGGCG2550.071.86994
TAGCGGG9500.071.724422
ACGGGAT5400.071.358024
AGAGGGC20200.070.953063
GTAAAGG11500.070.3733441
TAGAGGG30600.070.334222
CGAGGGA8650.070.0803453
AGGGCAT14150.069.742595
GAATAGG10700.069.478561
ATAGAGG10850.069.3853451
ATAGGGC9100.069.19683
AAGGGTA9800.069.049264