FastQCFastQC Report
Mon 27 Feb 2023
SRR3126895.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126895.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111469
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57630.5185029901868609No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36980.3327128331964274No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC30570.2750414091621089Illumina Single End Adapter 1 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28920.26019619080694106No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG26520.238603145926697No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG23860.2146708545177598No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT22780.20495398432165002No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG20970.18866922964113259No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT16100.144853342738304No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCAGCAAGATCGT14110.12694910969176829No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT201.8392722E-593.985568
CGTAGGG3500.087.571712
AGTAAGG6400.086.979451
GCTACGA5850.085.441444
AGCGGGA6600.085.020083
CGCTACG5900.084.717353
CGTTTTT29350.083.259971
CTACGAC6000.083.020585
AGGGCGT1200.082.2373665
ATAGCGG1700.080.47551
AGGGATG20950.079.854085
CGGGATA1650.079.745325
TACGACC6250.079.699756
TAGGGCG1900.079.417264
AAGGGTA5700.079.417254
CGAGGGA5550.079.014813
AGGGCAT10000.078.947875
GGATGGC13900.078.772077
TAATCGG301.4242305E-678.6254961
ATTGCGG1150.077.941791
Produced by FastQC (version 0.11.5)