FastQCFastQC Report
Mon 27 Feb 2023
SRR3126894.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126894.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115874
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55690.49907068360764745No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35560.3186739721509776No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC31070.27843645429501895Illumina Single End Adapter 1 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29380.2632913751911058No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG27430.24581628391735985No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG22940.2055787660614012No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT21650.1940183210649231No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21190.18989599184137276No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCAGCAAGA20320.1820994126577015No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT16390.14688038255215194No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCAGCAAGATCGT13080.11721753531312674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAAGG7200.084.3214951
CGTTTTT29400.083.080881
ATAGCGG2050.082.64751
TCCGCTA6800.082.360521
AAGGGTA4950.081.652724
CGCTACG6750.081.462823
GCTACGA6800.080.863834
CGTAGGG3100.080.41232
CTACGAC6850.080.273585
CGAAGGG9450.079.633622
AGGGTAT4950.078.804375
TCACGGG1200.078.389352
AGGGCAT9000.078.3296365
AGGGATG19000.078.164735
ACGGGTA1450.077.789434
AACGAGG4250.077.5157851
TACGACC7100.077.447056
AGACGGG6400.077.164512
TGTAGGG7550.076.624292
AGAGGGC11350.076.604323