FastQCFastQC Report
Mon 27 Feb 2023
SRR3126893.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126893.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159592
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58950.5083684606309805No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36340.31338608752043823No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC34590.2982945725738018Illumina Single End Adapter 1 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29880.2576768380602833No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG29670.2558658562666869No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG26670.2299946877867388No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT23940.206451924469986No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG21590.18618617582735997No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT17910.15445087582529027No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCAGCAAGA14900.12849347011707565No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCAGCAAGATCGT14380.124009134247218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGG1750.086.046181
CGAAGGG10400.085.846282
CGTAGGG3750.085.20772
CGGGATA1450.084.257115
GCTACGA5800.084.257114
CGTTTTT29150.084.1044161
AGGGCGT1900.084.0865555
CGCTACG5900.082.829033
AGGGTAT4900.082.471445
TAGGGCG2350.081.9817664
GAGGGTA4200.081.67234
CTAACGG750.081.564611
ATTGCGG1500.081.564611
CTACGAC6000.081.448555
AGGGATG18150.081.034325
TCCGCTA6050.080.890521
TACGGGA2450.080.5535053
TACGACC6150.079.4626
TAGCGGG5450.079.32182
TAAACGG1250.079.054931
Produced by FastQC (version 0.11.5)