Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126892.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1815054 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 48687 | 2.6823995319147533 | No Hit |
CGTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT | 5387 | 0.29679557743185603 | No Hit |
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT | 4502 | 0.24803669753076218 | RNA PCR Primer, Index 38 (95% over 21bp) |
CGTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT | 4022 | 0.22159120334711802 | No Hit |
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG | 3885 | 0.21404321854886962 | RNA PCR Primer, Index 38 (95% over 22bp) |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 2868 | 0.15801182774727363 | No Hit |
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC | 2570 | 0.14159358344159456 | RNA PCR Primer, Index 38 (95% over 23bp) |
CGTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC | 2239 | 0.12335721141078998 | No Hit |
CGTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC | 2078 | 0.11448695190335934 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 16805 | 0.0 | 90.32968 | 1 |
ATAGCGG | 335 | 0.0 | 77.470406 | 1 |
AGGGTAC | 1235 | 0.0 | 75.72125 | 5 |
TAGGGTC | 780 | 0.0 | 74.94192 | 4 |
ATAGGGC | 1170 | 0.0 | 73.73317 | 3 |
ACGGGAT | 475 | 0.0 | 73.44052 | 4 |
AGAGGGT | 2615 | 0.0 | 73.00978 | 3 |
AAGGGTA | 1965 | 0.0 | 72.93041 | 4 |
GAGGGTA | 1365 | 0.0 | 72.17908 | 4 |
TAGGGTA | 880 | 0.0 | 71.7827 | 4 |
CGTAGGG | 555 | 0.0 | 71.35033 | 2 |
AGGGTAT | 1515 | 0.0 | 71.03208 | 5 |
AAGGGTC | 2080 | 0.0 | 70.71132 | 4 |
GTAGGGT | 870 | 0.0 | 70.4404 | 3 |
ACGGGTA | 395 | 0.0 | 70.41296 | 4 |
TAAGGGA | 1825 | 0.0 | 70.00098 | 3 |
ATAGGGT | 1160 | 0.0 | 69.492165 | 3 |
ACGGGTC | 335 | 0.0 | 68.95233 | 4 |
AGGGTGC | 1180 | 0.0 | 68.89628 | 5 |
ATAAGGG | 2430 | 0.0 | 68.28826 | 2 |