FastQCFastQC Report
Mon 27 Feb 2023
SRR3126892.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126892.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815054
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT486872.6823995319147533No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT53870.29679557743185603No Hit
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT45020.24803669753076218RNA PCR Primer, Index 38 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT40220.22159120334711802No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG38850.21404321854886962RNA PCR Primer, Index 38 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28680.15801182774727363No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC25700.14159358344159456RNA PCR Primer, Index 38 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC22390.12335721141078998No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC20780.11448695190335934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT168050.090.329681
ATAGCGG3350.077.4704061
AGGGTAC12350.075.721255
TAGGGTC7800.074.941924
ATAGGGC11700.073.733173
ACGGGAT4750.073.440524
AGAGGGT26150.073.009783
AAGGGTA19650.072.930414
GAGGGTA13650.072.179084
TAGGGTA8800.071.78274
CGTAGGG5550.071.350332
AGGGTAT15150.071.032085
AAGGGTC20800.070.711324
GTAGGGT8700.070.44043
ACGGGTA3950.070.412964
TAAGGGA18250.070.000983
ATAGGGT11600.069.4921653
ACGGGTC3350.068.952334
AGGGTGC11800.068.896285
ATAAGGG24300.068.288262