Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126891.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1812863 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 46378 | 2.558273846396556 | No Hit |
CGTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT | 5323 | 0.2936239528304125 | No Hit |
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT | 4416 | 0.24359259359366925 | RNA PCR Primer, Index 38 (95% over 21bp) |
CGTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT | 3999 | 0.22059030384535402 | No Hit |
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG | 3881 | 0.21408126262161012 | RNA PCR Primer, Index 38 (95% over 22bp) |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 2798 | 0.154341502915554 | No Hit |
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC | 2654 | 0.14639826616793436 | RNA PCR Primer, Index 38 (95% over 23bp) |
CGTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC | 2112 | 0.11650080563175487 | No Hit |
CGTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC | 2103 | 0.11600435333502862 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 16055 | 0.0 | 89.286255 | 1 |
ACGGGAT | 410 | 0.0 | 79.09194 | 4 |
ACGGGTA | 275 | 0.0 | 76.903625 | 4 |
AGGGTAC | 1205 | 0.0 | 76.4427 | 5 |
AAGGGTA | 1785 | 0.0 | 73.98354 | 4 |
AGAGGGT | 2610 | 0.0 | 73.82617 | 3 |
TAGGGTC | 780 | 0.0 | 73.5076 | 4 |
ACGGGTC | 320 | 0.0 | 73.43229 | 4 |
ATAGCGG | 395 | 0.0 | 72.653275 | 1 |
AGGGATG | 2580 | 0.0 | 72.49873 | 5 |
AAGGGTC | 1900 | 0.0 | 72.4738 | 4 |
AGGGTAT | 1495 | 0.0 | 71.67384 | 5 |
GAGGGTA | 1440 | 0.0 | 71.14773 | 4 |
TAGGGTA | 1050 | 0.0 | 70.71879 | 4 |
TAACGGG | 560 | 0.0 | 70.52417 | 2 |
TACGGGT | 240 | 0.0 | 70.494995 | 3 |
ATAGGGT | 1420 | 0.0 | 70.16403 | 3 |
CGAAGGG | 1700 | 0.0 | 69.69447 | 2 |
AGAGGGC | 2530 | 0.0 | 69.65909 | 3 |
TAGCGGG | 1000 | 0.0 | 68.64353 | 2 |