FastQCFastQC Report
Mon 27 Feb 2023
SRR3126891.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126891.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812863
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT463782.558273846396556No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT53230.2936239528304125No Hit
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT44160.24359259359366925RNA PCR Primer, Index 38 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT39990.22059030384535402No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG38810.21408126262161012RNA PCR Primer, Index 38 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27980.154341502915554No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC26540.14639826616793436RNA PCR Primer, Index 38 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC21120.11650080563175487No Hit
CGTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC21030.11600435333502862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT160550.089.2862551
ACGGGAT4100.079.091944
ACGGGTA2750.076.9036254
AGGGTAC12050.076.44275
AAGGGTA17850.073.983544
AGAGGGT26100.073.826173
TAGGGTC7800.073.50764
ACGGGTC3200.073.432294
ATAGCGG3950.072.6532751
AGGGATG25800.072.498735
AAGGGTC19000.072.47384
AGGGTAT14950.071.673845
GAGGGTA14400.071.147734
TAGGGTA10500.070.718794
TAACGGG5600.070.524172
TACGGGT2400.070.4949953
ATAGGGT14200.070.164033
CGAAGGG17000.069.694472
AGAGGGC25300.069.659093
TAGCGGG10000.068.643532