Basic Statistics
Measure | Value |
---|---|
Filename | SRR3126890.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1896753 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 48439 | 2.553785337363378 | No Hit |
CGTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT | 5670 | 0.298931911535134 | No Hit |
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT | 4933 | 0.2600760352033185 | RNA PCR Primer, Index 38 (95% over 21bp) |
CGTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT | 4304 | 0.22691410004360082 | No Hit |
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG | 4176 | 0.22016572532111456 | RNA PCR Primer, Index 38 (95% over 22bp) |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 2913 | 0.15357824661408206 | No Hit |
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC | 2873 | 0.1514693795133051 | RNA PCR Primer, Index 38 (95% over 23bp) |
CGTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC | 2230 | 0.11756934086831548 | No Hit |
CGTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC | 2186 | 0.11524958705746081 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 16900 | 0.0 | 90.27148 | 1 |
AGGGTAC | 1085 | 0.0 | 76.23551 | 5 |
AAGGGTC | 2020 | 0.0 | 75.38206 | 4 |
GTACGGG | 425 | 0.0 | 74.08816 | 2 |
AGGGTAT | 1625 | 0.0 | 74.03907 | 5 |
GTAGGGT | 975 | 0.0 | 73.74791 | 3 |
ACGGGAT | 480 | 0.0 | 73.433525 | 4 |
TAGCGGG | 1060 | 0.0 | 73.154495 | 2 |
AAGGGTA | 1980 | 0.0 | 72.86979 | 4 |
ATAGCGG | 460 | 0.0 | 72.631454 | 1 |
TAGGGTC | 830 | 0.0 | 71.91177 | 4 |
AGGGATG | 2655 | 0.0 | 71.69103 | 5 |
GAGGGTA | 1520 | 0.0 | 71.42377 | 4 |
AGAGGGT | 2765 | 0.0 | 71.04672 | 3 |
GGGTACG | 200 | 0.0 | 70.496185 | 6 |
ATAAGGG | 2440 | 0.0 | 70.30172 | 2 |
GGGCGAT | 840 | 0.0 | 69.93668 | 6 |
ATGCGGG | 820 | 0.0 | 69.9212 | 2 |
TAGGGCG | 345 | 0.0 | 69.4745 | 4 |
TAAGGGT | 1360 | 0.0 | 69.11208 | 3 |