FastQCFastQC Report
Mon 27 Feb 2023
SRR3126890.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126890.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896753
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT484392.553785337363378No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT56700.298931911535134No Hit
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT49330.2600760352033185RNA PCR Primer, Index 38 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT43040.22691410004360082No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG41760.22016572532111456RNA PCR Primer, Index 38 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29130.15357824661408206No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC28730.1514693795133051RNA PCR Primer, Index 38 (95% over 23bp)
CGTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC22300.11756934086831548No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTC21860.11524958705746081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT169000.090.271481
AGGGTAC10850.076.235515
AAGGGTC20200.075.382064
GTACGGG4250.074.088162
AGGGTAT16250.074.039075
GTAGGGT9750.073.747913
ACGGGAT4800.073.4335254
TAGCGGG10600.073.1544952
AAGGGTA19800.072.869794
ATAGCGG4600.072.6314541
TAGGGTC8300.071.911774
AGGGATG26550.071.691035
GAGGGTA15200.071.423774
AGAGGGT27650.071.046723
GGGTACG2000.070.4961856
ATAAGGG24400.070.301722
GGGCGAT8400.069.936686
ATGCGGG8200.069.92122
TAGGGCG3450.069.47454
TAAGGGT13600.069.112083