FastQCFastQC Report
Mon 27 Feb 2023
SRR3126887.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126887.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962933
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106830.5442366091965442No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA49100.2501358935837341No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29550.15054003371485425No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG28710.1462607231117924No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26890.13698888347182508No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG25200.1283793180918554No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT20860.10626954664270252No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG19660.10015624578118561No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT45950.081.819811
ATAGCGG5000.080.9331
TAGCGGG11650.076.646712
AGGGCAT19600.076.249485
CGAAGGG18650.075.849642
TAGGGCG5100.075.563064
TACGGGT2400.074.411183
AGGGATG22500.073.9412165
AGGGCGA10350.073.559795
TAGGGCA12950.071.4928054
ACGGGTA1850.071.129894
ACGGGAT4650.070.7474754
AGTACGG2200.070.58111
GTACGGG3750.070.1814962
AAGGGTA12150.069.62454
AGAAGGG67250.069.394142
AGTAGGG29500.069.140662
GTAGGGC8850.069.0344543
GGTAAGG11900.068.801751
ATAGGGC16200.068.7541963
Produced by FastQC (version 0.11.5)