FastQCFastQC Report
Mon 27 Feb 2023
SRR3126886.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126886.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747531
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39560.5292088221090497No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33350.4461353442198384No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG23410.3131642701105372No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC21050.2815936730383088No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG20330.27196196545695095No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG19510.26099252071151563No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT17510.23423777742996613No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGT11990.16039468597288942No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA10060.1345763587061941No Hit
AGAGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT9050.1210652133490116No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT8950.11972747618493414No Hit
GGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATCTCT8170.10929312630512983No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT8150.10902557887231433No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT7680.10273821420115017No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG201.7990718E-594.40071
TATCGGG156.800064E-494.349982
GGCGTAC156.905949E-493.983948
TCGCGAT156.905949E-493.9839494
TAACGGG2150.092.15582
ATTGCGG900.089.156211
CGTTTTT23300.087.715671
GTAGCGG1400.087.657791
CGTAGGG2000.087.2737352
AGGGTAG2700.087.022185
TCGAGGG3150.086.861892
GTACCGA550.085.439968
GCATAGG1550.085.2651441
CTAACGG500.084.9606251
AGGGCGC1000.084.585555
TAGCAGG2350.084.358071
AAGGGTA4200.084.241064
TAGTAGG3250.084.234471
ACGGGAT850.083.2499854
CGAAGGG5800.082.962922
Produced by FastQC (version 0.11.5)