FastQCFastQC Report
Mon 27 Feb 2023
SRR3126885.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126885.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753351
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39110.5191471173463631No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32470.43100759141489164No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG22050.2926922510224318No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC21540.2859224982776953No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG19550.2595071885482332No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG18210.2417199950620627No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGCAAGCG17080.22672034682372494No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT16500.21902141232971084No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGT11730.1557043131289399No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA9860.13088188639823933No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT8570.11375839416155285No Hit
AGAGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT8480.11256373191248169No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT8280.1099089269145458No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT7750.1028736936700157No Hit
GGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATCTCT7620.10114807042135737No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACG201.8377605E-593.997156
ACGTAGG1050.089.59841
ATGACGG800.088.1984251
ACGGGAT1500.087.730674
GTAGGGT2350.085.997383
CGAAGGG5800.085.128822
GGGCGAT2550.084.7817466
ATTACGG500.084.670491
CTAGCGG500.084.670491
CGTTTTT22850.084.6087341
AGGGCAT5000.084.5974355
AGCGGGT1800.083.553023
TAGCAGG2200.083.3875961
GTACAGG2150.083.138981
CGGGATA850.082.938665
TGCGGGG4750.082.1238253
CGTAGGG2350.082.041072
AGGGCAC3000.081.464195
ATAGCGG1450.081.1020051
CAGGGCA2850.080.8045654
Produced by FastQC (version 0.11.5)