FastQCFastQC Report
Mon 27 Feb 2023
SRR3126884.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126884.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796489
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40150.504087313195788No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34000.42687344081336964No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG25550.32078283567004695No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC23070.28964618469307174No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG22530.2828664300448594No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG20240.2541152482959589No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT17390.2183332098748382No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGT13240.1662295398932063No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGCAAGCG12440.15618545893289174No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA10570.13270741968815639No Hit
AGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT9740.12228668569183002No Hit
AGAGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT9450.11864570634371598No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT9020.1132470128275469No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT8510.10684391121534637No Hit
GGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATCTCT8390.10533729907129916No Hit
GGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGCGGTCA8010.10056636061514974No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG254.888934E-794.099371
ATTGCGG950.094.0993651
CGGGTAC156.9051853E-493.987135
CGTAGGG2600.090.372252
CGTTTTT23800.087.9710541
ACGGGTA750.087.721334
TAGCGGG3400.087.076322
GTCGAGG1100.085.544881
ATAGCGG1500.084.689441
CGAAGGG7500.084.588422
GTAGGGT2450.084.3966143
CGAGGGA2900.084.264333
TAACGGG1900.084.093752
ACGGGAT1400.083.9170764
AGGGCAT6750.083.544115
GTAAAGG4900.083.53721
GACACGG850.083.0288541
ACGTAGG1650.082.693381
GCGTAGG800.082.336951
GGTAAGG4400.082.3369451
Produced by FastQC (version 0.11.5)