FastQCFastQC Report
Mon 27 Feb 2023
SRR3126883.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126883.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180479
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181420.8320190196741175No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39160.1795935663677568No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34960.16033174362147032No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26190.12011122326791498No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25720.11795573357964008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70350.085.2526551
AGGGTAC10150.079.6329045
ATAGGGC13350.074.522563
CGAAGGG21900.073.417042
ATAGCGG5300.073.006991
AGGGATG29600.072.235375
AAGGGTA14550.070.6447754
CGTAGGG6350.070.540162
ATGTAGG7700.069.861931
AGAGGGC30900.069.123593
GGTAAGG13900.068.914031
TAGAGGG40500.068.3391652
AGGGCAT17300.068.180125
AGTAAGG12400.068.117361
GAATAGG14200.067.790411
TAGGGTA6200.067.683834
TAGCGGG12900.067.618982
ATAGGGA20450.067.5555343
AGTAGGG35400.067.395952
AAGGGCA32100.066.8333054
Produced by FastQC (version 0.11.5)