FastQCFastQC Report
Mon 27 Feb 2023
SRR3126882.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126882.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182545
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174970.8016787740917141No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT39070.1790112002272576No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33670.1542694423253587No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26350.12073061494722905No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT25150.11523244652458482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71000.085.588191
AGGGATG25150.074.3744665
AGGGTAC9450.073.605245
CGAAGGG21250.073.040252
CGTAGGG5950.072.724072
ATAGCGG4900.072.046211
ACGGGAT4550.071.27164
ATAGGGC12900.069.221773
AGTAAGG12800.068.76661
AAGGGTA14500.068.714234
ACGGGTA1850.068.591584
GAATAGG13700.068.028441
AGTAGGG34750.067.538752
AGAGGGC28300.067.424613
AGAAGGG81800.066.985362
ATAGAGG14200.066.9591
TAGCGGG12400.066.7572252
TAAGGGA19150.066.754253
GGTAAGG12100.066.520681
ATAGGGA19800.066.2243653
Produced by FastQC (version 0.11.5)