FastQCFastQC Report
Mon 27 Feb 2023
SRR3126879.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126879.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379000
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268211.1274064733081128No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42690.1794451450189155No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31160.13097940311055067No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29050.12211013030685162No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG27270.11462799495586382No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC27150.1141235813366961No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT25200.10592686002522068No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT24900.10466582597730138No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG24030.10100882723833543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95950.086.4925461
ACGGGAT7700.076.2951354
AGGGATG35350.074.9837655
TAAGGGA19650.074.622473
ATAGAGG15550.073.821781
AGTACGG3000.073.7057951
ACGGGTA2650.072.713584
ATAGGGC15800.072.281433
GGTAAGG15900.071.900881
CGAAGGG22200.071.170712
AGGGCAT20800.069.818855
AGAGGGC34250.069.433263
AGTAGGG41250.069.423782
TGTAGGG18000.069.229042
GGGCGAT11400.068.847666
AGGGCGA14200.068.510885
TAGAGGG44050.068.213432
TAGGGCA14750.068.1866464
AGACGGG14450.068.013222
GTAGGGC11800.067.70873
Produced by FastQC (version 0.11.5)