FastQCFastQC Report
Mon 27 Feb 2023
SRR3126878.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126878.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528215
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279631.1060372634447624No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41800.16533404002428592No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33480.13242544641179646No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC30410.1202824917975726No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA30300.11984740221856131No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG29450.1164853463807469No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT27980.11067096746123252No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG27320.10806042998716485No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26540.10497524933599396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106250.085.861561
ACGGGAT8100.077.751014
AGGGATG40450.073.5480965
ATAGGGC15950.073.369633
ACGGGTA2800.072.176644
AGAGGGC34300.071.250333
TAAGGGA22700.070.807283
ATAGGGA24450.070.544783
GTAGGGC10800.070.496733
TATAGGG18050.070.041072
TAGGGCA12900.069.951624
TAGGGAC12700.069.9430164
GAATAGG16700.069.623271
AAGGGTA13750.069.0454254
AGTAAGG15900.068.685461
TAGAGGG46050.068.685192
AGTAGGG42250.068.63352
AAGGGAC21450.068.361814
ATAGAGG17700.068.0833051
GGTAAGG15450.067.943881