FastQCFastQC Report
Mon 27 Feb 2023
SRR3126877.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126877.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159060
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290471.3453539966466888No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38560.17859624095671264No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36840.17062981112150657No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT23920.11078895445240058No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT22570.10453623336081444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100650.087.8842541
CGAAGGG19250.074.705142
ATAGCGG3750.072.9659651
TAGGGCG5250.072.745664
AGTACGG3050.072.697861
TAGGGCA11250.072.087064
AGGGCAT19850.071.495245
ATAGGGC13400.071.428683
AGGGTAC10000.070.959025
GTACGGG5400.069.85182
TAGAGGG36900.069.766612
TAGCGGG11050.069.551542
ACGGGTA1700.069.338184
CGTAGGG6600.069.296162
ATAGAGG16150.068.9387051
GTAGGGC9100.068.911483
AGAGGGC28100.068.459733
TGTAGGG14800.068.17632
GGGCGAT10300.067.9797746
AATAGGG33500.067.558162
Produced by FastQC (version 0.11.5)