FastQCFastQC Report
Mon 27 Feb 2023
SRR3126876.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126876.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160069
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272311.260654173547234No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38760.17943871237446582No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36150.16735576502417282No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT22560.10444110813126802No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT22250.10300596879081177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96000.087.16951
ACGGGTA2450.080.562584
CGAAGGG21950.071.768182
ATAGGGC13200.071.5603263
ATAGCGG4650.070.8515241
GGTAAGG12100.070.792971
AGGGTAC10550.070.38095
CGTAGGG5200.069.6320342
AGTAAGG14850.068.776071
TAGCGGG11350.068.775522
AGTAGGG37450.068.684252
GTACGGG4800.068.5769962
AGGGCAT18100.068.544955
TAGGGCA9900.068.3561254
AGAGGGC31300.068.165043
AAGGGTA13550.067.977774
GTAGGGC11350.067.9044343
TAGGGTC6250.067.672574
AGGGATG25450.067.029975
AGTACGG2250.066.937821
Produced by FastQC (version 0.11.5)