FastQCFastQC Report
Mon 27 Feb 2023
SRR3126875.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126875.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268399
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288621.2723511163600407No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38680.17051673889822733No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA38450.1695028079275295No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT25310.11157649073200966No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT23700.10447897393712482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT100550.087.279241
ACGGGTA2550.075.5618064
GTACGGG5000.073.313382
CGAAGGG22400.071.752452
ATAGGGC12950.071.128713
AGGGCAT19650.071.031755
TAGACGG2850.070.996651
ATAGAGG15450.069.4417341
AGTAAGG15950.069.330031
AGAGGGC32050.068.7707063
TAGCGGG10450.068.357472
TAGAGGG38500.068.23542
TAGGGCG4350.068.062824
AGGGCGA11300.067.7903445
ATGAGGG36050.067.658252
TACGAGG5150.067.614331
AATGCGG6550.067.530631
ATAGCGG5100.067.3545461
AGGGCAC15650.067.2654955
AAGAGGG75250.066.949442
Produced by FastQC (version 0.11.5)