FastQCFastQC Report
Mon 27 Feb 2023
SRR3126874.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126874.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295908
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158170.6889213330847752No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42430.18480705672875394No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42260.18406660894077637No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT31690.1380281788294653No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG28760.12526634342491075No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28610.12461300714140114No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG26540.1155969664289684No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC24650.10736492925674723No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG23560.10261735226324399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61550.082.999351
ACGGGTA3050.078.829434
ATAGGGC13650.074.2546843
TAGCGGG10100.074.215362
AGTAGGG39000.073.8575062
AAGGGTA17000.073.4877554
AGGGATG28250.073.0265965
AGGGCAT21950.071.506735
CGTAGGG6650.070.891862
AGGGTAC11750.070.789745
AGTACGG3350.070.404351
TAGGGTC6650.070.1829454
TAAGGGA21400.070.053753
CGAAGGG22150.069.597252
GTACGGG5450.069.200862
AGTAAGG15550.069.163791
AGAGGGC31300.068.681313
GGTAAGG14600.068.494751
AAGGGAC21200.068.4909064
TAGAGGG38000.068.481542
Produced by FastQC (version 0.11.5)