FastQCFastQC Report
Mon 27 Feb 2023
SRR3126872.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126872.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2406764
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159130.6611782459767555No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42900.1782476387381563No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA42880.17816453960587744No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT31930.13266776468320118No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG31280.1299670428841382No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29800.12381770709550251No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG28930.12020289484137206No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC27460.1140951086188758No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG24330.10109009441723409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58200.083.275581
ACGGGTA2850.077.5039444
CGTAGGG6700.073.65062
TACGGGT1750.072.508263
TAGGGTC7150.071.645894
AGGGAAT28900.071.3914955
AGGGATG27700.071.2605745
AGTAGGG39150.071.184382
AAGGGTA17750.070.694194
TAGGGCG5150.070.267474
GAATAGG14000.069.909911
ATAGGGC14600.069.850363
GTAGGGT8450.069.520853
CGAAGGG22200.069.2239762
AGGGTAC12950.068.954665
AGGGCAT20900.068.585665
TAGCGGG10900.068.122782
AATAGGG33450.067.719342
AGAGGGC32150.067.387873
AAGGGAC23650.067.365784