FastQCFastQC Report
Mon 27 Feb 2023
SRR3126871.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126871.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251645
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72150.5764414031135027No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA59570.47593367128858416No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39810.3180614311565979No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT30330.24232110542526034No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG27180.21715422503984758No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG25730.205569470576721No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC23150.18495659711819246No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22740.18168090792517047No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT15510.12391692532627062No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA14600.11664649321492915No Hit
AAAGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT12600.10066752154165119No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG2050.085.078694
CGTTTTT29900.085.03621
GGTAAGG6400.084.7625661
ACGGGTA1400.084.175344
CGAAGGG10150.081.275992
CGCTACG8500.080.966773
AGTACGG2050.080.5379261
GCTACGA8450.080.330174
AGGGATG20750.080.171515
TCCGCTA8600.079.5345461
CTACGAC8650.078.7747045
CTAACGG900.078.620351
TACGGGT900.078.5636443
TAAGGGA10850.078.20163
CGACCAA8450.077.8585368
ATAGAGG8650.077.438781
AGACGGG7900.076.378862
TAGCGGG5500.076.281212
TGTAGGG9300.075.5255052
AGGGATT12950.075.116525