FastQCFastQC Report
Mon 27 Feb 2023
SRR3126869.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126869.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308474
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71950.5498771851790712No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA61040.46649761477874224No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC40350.30837448814420465No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG31860.2434897445421155No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT30300.23156745949862206No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG29350.22430709360675105No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC25510.19495993042276727No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23070.17631225381627758No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14850.11349098262556229No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA14360.10974616232343937No Hit
AAAGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTT14240.10882906347393986No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTC201.838597E-593.9937749
GGTAAGG5650.087.452971
TAGGGCG2600.086.763484
CGTTTTT30750.084.321861
CGTAGGG4100.083.6773762
ACGGGTA1300.083.148344
TACGGGT850.082.935683
CGAAGGG10950.081.5471042
AGTAAGG6500.079.636661
TCCGCTA8700.078.970931
ATTGCGG1550.078.9360351
GCATAGG3700.078.8541
AGGGATG21800.078.687455
AAGGGAC11150.077.97694
ACGGGAT3350.077.1590654
CGCTACG8850.077.000553
GCTACGA8800.076.369944
AGACGGG7850.076.033182
CTACGAC8850.075.938475
AGGGATT12750.075.932225
Produced by FastQC (version 0.11.5)