FastQCFastQC Report
Mon 27 Feb 2023
SRR3126868.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126868.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253228
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216320.9600448778374846No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53650.23810284622772307No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31350.1391337228189957No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24360.1081115626115067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74200.086.323971
ATAGGGC15250.078.559743
TAGGGCA10750.075.0280764
TAGGGTA6050.073.283914
AGTAGGG36150.071.893422
GCATAGG6000.070.01551
TAGCGGG12150.069.490042
AGGGCAT18000.069.44375
CGTAGGG6250.069.431042
AGTAAGG12700.068.758041
AGGGATG26100.068.237395
GAATAGG14600.068.215811
AATAGGG34350.067.833782
ATAGAGG16850.067.7908251
TATAGGG17500.067.652132
TAACGGG7000.067.382612
TAGAGGG46150.067.35342
TAAGGGA17300.067.3421553
ACGAGGG22300.067.2617342
GGTAAGG14050.066.8547441
Produced by FastQC (version 0.11.5)