FastQCFastQC Report
Mon 27 Feb 2023
SRR3126867.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126867.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250271
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211630.9404645040530674No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT54130.24054880501059647No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29430.130784247763936No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG23900.10620942988644479No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC22790.10127669067414548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76600.085.269641
ATAGGGC13100.077.1313863
AGTAAGG11350.074.214861
CGTAGGG6750.073.149292
ACGGGAT4500.072.061044
GTAGGGT7150.071.644763
GAATAGG14400.070.9138261
AGTAGGG33400.070.536812
ATAGAGG16150.070.514191
AGGGATG22900.069.365775
ATAGGGA18750.068.426663
AAGGGTA14500.068.3877644
GCATAGG6200.068.3100361
GGTAAGG13350.068.031451
TAGGGTA5350.067.639594
TAGGGCA11050.067.623684
TAAGGGA16600.067.380283
AAGAGGG76450.067.292152
AGAAGGG80300.067.228112
AATAGGG32700.066.869772
Produced by FastQC (version 0.11.5)