FastQCFastQC Report
Mon 27 Feb 2023
SRR3126866.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126866.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334194
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220520.9447372412061722No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT53890.23087198407673054No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32140.1376920684398983No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC25040.10727471666879444No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG24870.10654641387990886No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77450.085.961081
ATAGGGC13350.073.221223
AGGGATG27500.071.4365
TAGGGTA5200.071.399854
ATAACGG2250.071.098961
AGTAGGG37750.070.212922
TAGGGCA11250.070.182734
GCATAGG6650.070.045531
GGTAAGG14250.068.677641
CGTAGGG7800.068.083012
TAAGGGA19700.067.9882053
ACGGGAT4850.067.8312454
GAATAGG12250.067.5994951
AGGGCAT18600.066.958615
TAGTAGG8800.066.83351
TAGAGGG46600.066.559942
AGGGTAC9450.066.144445
AGGGAAT26200.065.832195
ATAGAGG15800.065.811541
ACGGGTA2000.065.796314
Produced by FastQC (version 0.11.5)