FastQCFastQC Report
Mon 27 Feb 2023
SRR3126865.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126865.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034211
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133720.657355603720558No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41540.20420693821830674No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40070.19698054921539607No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT24950.12265197661402874No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG20960.10303749217755681No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55550.083.0290151
ACGGGAT5450.074.377474
CGTAGGG4650.073.9961852
AGGGATG27000.072.056275
ATAGGGC14200.072.029573
GTACGGG6150.071.276652
TAGGGCA10400.071.155054
AGTAGGG35500.070.768232
ATAGCGG3950.070.441731
TAGGGCG3900.070.097554
CGAAGGG19550.069.677172
GGTAAGG13050.069.3850251
TAAGGGA18500.069.3005453
TAGGGTA7100.069.042174
TAGCGGG9850.068.4288562
AGAGGGC27750.068.111563
GTAGGGC9000.068.083253
TGTAGGG17550.067.948972
AGTACGG3550.067.751181
ATGAGGG30800.067.641152
Produced by FastQC (version 0.11.5)