FastQCFastQC Report
Mon 27 Feb 2023
SRR3126864.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126864.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037099
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125150.6143540397398457No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42230.20730460326179534No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39100.19193961609131416No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT23920.11742188278527455No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG20650.10136964379247156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48550.081.622451
ACGGGAT6050.075.35024
TAGGGCG3850.074.462424
AGTACGG2850.074.311221
ATAGCGG4800.073.537151
CGTAGGG5400.071.3987662
GGTAAGG14050.071.349351
TAGCGGG11250.071.0504762
CGAAGGG19500.069.925252
AGTAGGG33900.069.903912
AGGGCGA11800.069.300335
TAAGGGA18850.068.812253
AGAGGGC28150.068.616963
TACGGGT2200.068.358943
AGGGATG26300.068.2614755
GAATAGG14350.068.217881
GTACGGG5050.067.967632
AAGGGTA12850.067.660724
AGGGTAC10000.067.205385
CGAGGGA10650.067.07523
Produced by FastQC (version 0.11.5)