FastQCFastQC Report
Mon 27 Feb 2023
SRR3126863.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126863.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162694
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133200.6158985043653887No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41770.1931387426977649No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40530.18740515301748653No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26160.12096024680329255No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22290.1030658983656495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53100.084.22321
ACGGGTA1950.072.3028954
ATAGCGG4900.072.055621
CGTAGGG6600.071.91782
ATAGGGC14300.071.6439363
GAATAGG14800.071.250661
AGTAGGG35050.071.197622
ACGGGAT6300.070.868314
TAGGGCG4600.070.4953164
AGGGCGA11650.070.1927645
TAGCGGG10850.070.168842
AGGGATG29350.069.494535
GGTAAGG12400.068.716261
TAAGGGA19450.067.896243
AGGGCAT20450.067.565195
AAGGGTA14400.067.5580144
AGGGTAC9000.067.362195
TGTAGGG17350.067.1755452
AAGGGCG11650.066.56214
TAGGGCA11950.066.46424
Produced by FastQC (version 0.11.5)