FastQCFastQC Report
Mon 27 Feb 2023
SRR3126862.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126862.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2467350
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153640.6226923622509981No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47470.19239264798265346No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45240.18335461122256672No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27290.11060449470079235No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG25840.10472774434109469No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59850.083.820571
ACGGGAT6650.076.584184
CGTAGGG5850.073.355272
TAGGGCG4850.071.949364
AGTAGGG42000.071.745952
ATAGGGC16850.071.643543
CGAAGGG24100.070.63762
AGGGATG34550.070.59095
AGGGCAT22450.070.541225
TAGGGCA14300.070.239364
AGGGCGA13800.070.148375
TAAGGGA21500.069.527693
TAGCGGG13400.069.327772
AGAGGGC33250.068.9257663
TGTAGGG20700.068.799042
GGGCGAT13200.068.708876
GGTAAGG15800.068.6992951
GAATAGG14800.068.558021
TATAGGG20500.068.090052
AGTAAGG16650.067.743121
Produced by FastQC (version 0.11.5)