FastQCFastQC Report
Mon 27 Feb 2023
SRR3126861.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126861.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468348
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145610.5899087162750147No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT47070.1906943429370575No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44660.18093072775799846No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT27730.11234234394826012No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26720.10825053841678725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59050.084.164461
ACGGGAT6000.074.413144
CGTAGGG6550.072.516852
AGGGATG32400.072.237325
AGTAGGG41750.072.091222
GGGCGAT12800.071.598176
TAGGGCG5600.071.335914
CGAAGGG24950.069.9297942
ACGGGTA2700.069.626334
TAGGGCA13300.069.259874
ATAGGGC16450.069.139583
AAGGGTA15800.068.711944
GTACGGG5900.068.549662
GGTAAGG15250.068.2035751
TAACGGG7400.068.000312
TAGCGGG12350.067.781622
AGGGCAT21450.067.703095
CGAGGGA14650.067.048013
TATAGGG18200.066.924832
AAGGGAC25150.066.899414
Produced by FastQC (version 0.11.5)