FastQCFastQC Report
Mon 27 Feb 2023
SRR3126860.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126860.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564130
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153310.5979026024421539No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT48510.18918697569936002No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45460.17729210297449816No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT28610.11157780611747453No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG26350.10276390042626543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61250.081.743451
ACGGGAT7100.076.118314
CGTAGGG5750.075.191652
TAGGGCA13800.072.535434
AGGGATG33200.070.9168245
ATAGGGC16850.069.72523
TAGGGCG5800.069.681924
TAAGGGA23750.069.6512153
AGTAAGG17500.069.3743741
GAATAGG16100.069.269161
AGAGGGC35700.069.2416153
CGAAGGG24550.069.104342
TAGCGGG14450.068.9473652
GGGCGAT13300.068.902126
ATAGCGG5750.068.7430651
AGTAGGG43950.068.326882
ATAGAGG18100.068.114591
AACGAGG10750.067.848571
ACGGGTA2300.067.42734
AAGAGGG92500.067.113632
Produced by FastQC (version 0.11.5)