FastQCFastQC Report
Mon 27 Feb 2023
SRR3126859.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126859.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684596
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83550.4959646110996346No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC64770.38448387625282265No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA63410.37641072399554554No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC50590.3003093916879774No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG40850.2424913747865957No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG36520.21678788267335314No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG35870.2129293907856839No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT35020.20788367062488572No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT19740.11717943055783107No Hit
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT17140.10174546300715423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG5500.089.186482
ATAGCGG2300.086.1881261
CGTTTTT42650.085.322061
CGGGATA2250.083.5419165
CGCTACG11950.082.883253
ACGGGAT4200.081.9764254
CTAACGG1100.081.524271
GCTACGA12200.080.798234
AGTAAGG9550.080.558291
AGGGATG32250.080.433385
AGGGCAT15400.080.2531365
CGAAGGG14300.079.819252
TAGCGGG8000.079.002922
CTACGAC12650.078.382465
TCCGCTA12850.078.235251
TAGGGCG2050.078.2240754
GGTAAGG10650.077.9991
GGATGGC20700.077.6394967
AGAGGGT13500.077.210073
GGGCGAT6700.077.151586