FastQCFastQC Report
Mon 27 Feb 2023
SRR3126857.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126857.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743760
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83490.4787929531586916No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC67210.38543148139652245No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA62640.35922374638711746No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC55920.320686332981603No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG45450.2606436665596183No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTG39880.22870119741248796No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG36960.21195577373032984No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT35300.20243611506170575No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT21330.12232187915768224No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAGGAAT20820.1193971647474423No Hit
CGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT18250.1046588980134881No Hit
AGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT18150.10408542459971555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG6000.086.157152
ATAGCGG2850.085.858161
CGCTACG12150.085.480273
CGTTTTT39950.084.5728151
CTAACGG1750.083.35791
GCTACGA12300.082.9095464
CGAAGGG15000.082.3975752
AGGGATG32650.082.18935
AGTAAGG10500.082.013411
CTACGAC12450.081.912995
TCCGCTA13000.080.3586651
GGTAAGG9750.079.1520841
CGGGATA2150.078.691245
CGACCAA12100.078.456398
TACGACC12950.078.387416
AGTACGG1750.077.979971
TAGCGGG6700.077.857072
GCGTAGG3050.077.142421
GGATGGC20050.077.11597
TAACGGG5750.076.82632