FastQCFastQC Report
Mon 27 Feb 2023
SRR3126856.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126856.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372319
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189020.7967731152513637No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT70730.29814708730149697No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31620.1332873024243367No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG28310.11933471004531852No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT24620.10378030947777259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64700.084.7043841
CGTAGGG7650.081.3930052
ATAGCGG3850.077.242751
AGTACGG3750.076.785011
ACGGGAT6450.076.0585864
GTAGGGC13100.074.1772163
GGTAAGG12150.073.42831
TAACGGG7800.072.570692
ATAGGGC15350.071.9088063
TAAGGGA16450.071.401613
AGTAGGG38850.071.1510242
GAATAGG17550.071.007581
AAGGGAC22550.070.9133154
AGGGCAT20300.069.909265
ACGGGTA2300.069.7309654
AGAGGGC28900.069.205823
AGGGATG29350.069.006985
TAGGGAC13250.068.709384
GTACGGG6200.068.473952
ATGAGGG33850.068.422262
Produced by FastQC (version 0.11.5)