FastQCFastQC Report
Mon 27 Feb 2023
SRR3126855.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126855.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372741
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180060.7588691728258583No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT70400.2967032642837967No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31760.133853631728031No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG27650.11653189286146275No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT24450.1030453808485629No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64650.084.155211
CGTAGGG6100.077.84732
ATAGCGG4850.073.750261
AGTAGGG40500.073.253162
GTAGGGC11650.073.0165943
ACGGGTA2400.072.453514
AGTAAGG14650.071.9618151
TAGGGAC14700.071.294584
AGGGACT42050.070.635015
ACGGGAT5550.070.283614
ATAGAGG16750.070.245251
AAGGGAC21400.069.616864
TAACGGG7650.069.449482
AGGGCAT22350.069.391355
ATAACGG2850.069.357941
GGTAAGG14050.069.34031
ATAGGGC13000.069.049253
AGGGATG28400.069.0059745
AATGCGG6550.068.979761
GAATAGG16200.068.853351
Produced by FastQC (version 0.11.5)