FastQCFastQC Report
Mon 27 Feb 2023
SRR3126854.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126854.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504377
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187880.7502065383925823No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT70280.28062867531525804No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31770.12685789719359344No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG28670.11447956917029666No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT26250.1048164872940456No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG25330.10114291897745428RNA PCR Primer, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70450.083.83991
ACGGGAT7000.077.20664
CGTAGGG7100.076.117662
ATAGGGC15400.074.763783
AGGGATG30300.071.8113255
ATAGAGG18600.071.101931
TAGGGCG5250.070.716774
AGTAGGG42750.070.3541642
TAGGGCA12850.070.218694
TAGCGGG12050.070.199072
TAAGGGA18500.070.1105353
GGTAAGG14450.070.0258941
AGTACGG3700.069.959881
ATAGGGA22150.069.802033
AGTAAGG16150.069.6487661
GTAGGGC11950.069.6067353
TAGGGAC13650.068.857614
AATAGGG39000.068.44312
GTAGGGA20450.068.2513
TAACGGG9300.068.217642
Produced by FastQC (version 0.11.5)