FastQCFastQC Report
Mon 27 Feb 2023
SRR3126852.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3126852.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220304
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66270.543061401093498No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA49140.4026865436809189No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36710.30082667925369416No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG31360.2569851446852588No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG27610.22625509709056105No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC26140.21420891843343953No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG22860.18733037013727727No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT19940.163401906410206No Hit
GGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTTGCA15660.12832867875545764No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTCCAGGT14740.12078957374555849No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT13220.10833366112050768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT31800.085.945471
ATAGCGG2300.085.900461
ACGGGAT4500.084.593514
CTACGAC5350.083.4515465
TCCGCTA5500.082.962731
AGGGATG20450.082.04265
AGCGGGA7850.082.019183
CGCTACG5450.081.920323
CATGCGG700.080.641241
ACGGGTA1350.080.067944
AGGGCGT1350.080.067945
AGGGCAT11650.079.87375
CGTAGGG3650.079.865492
CGGGATA2650.079.80525
CGAGGGA7100.079.430533
CGAAGGG12050.079.208052
AGGGCGA7550.078.431075
GCTACGA5700.078.327334
TTACGGG2050.077.980392
CACGGGC551.8189894E-1276.90323
Produced by FastQC (version 0.11.5)